Comparative Transcriptome Analysis Identifies MAPK Signaling Pathway Associated with Regulating Ovarian Lipid Metabolism during Vitellogenesis in the Mud Crab, Scylla paramamosain

The mud crab, Scylla paramamosain, has abundant nutrients in the ovary, where numerous lipids accumulate during ovarian maturation. However, mechanism behind accumulation of ovary crab maturation is largely unknown. This study conducted a comparative transcriptome analysis ovaries crabs at various stages A total 63.69 Gb clean data was obtained, with Q30 93.34%, and 81,893 unigenes were identified, including 10,996 differentially expressed genes (DEGs). After KEGG enrichment these DEGs, MAPK signaling pathway significantly enriched vitellogenesis. Moreover, expression levels involved carbohydrate, amino acid, lipid metabolism found to be higher two (Sp-Eip75B Sp-Eip78C) that are homologous vertebrate gene PPARγ PPAR pathway, identified. Additionally, might regulate through based on Protein-Protein Interaction (PPI) network. These findings suggest plays critical role vitellogenesis, which provides new insights into crabs.